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KMID : 0811720080120030111
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Korean Journal of Physiology & Pharmacology
2008 Volume.12 No. 3 p.111 ~ p.116
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(-)-Epigallocatechin Gallate Inhibits the Pacemaker Activity of Interstitial Cells of Cajal of Mouse Small Intestine
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Kim Kweon-Young
Choi Soo-Jin
Yeum Cheol-Ho
Choi Seok
Yoon Pyung-Jin
Jun Jae-Yeoul
Jang Hyuk-Jin
Zuo Dong-Chuan
Pawan Kumar Shahi
Shankar Prasad Parajuli
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Abstract
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The effects of (?)-epigallocatechin gallate (EGCG) on pacemaker activities of cultured interstitial cells of Cajal (ICC) from murine small intestine were investigated using whole-cell patch-clamp technique at 30oC and Ca2＋ image analysis. ICC generated spontaneous pacemaker currents at a holding potential of ?70 mV. The treatment of ICC with EGCG resulted in a dose-dependent decrease in the frequency and amplitude of pacemaker currents. SQ-22536, an adenylate cyclase inhibitor, and ODQ, a guanylate cyclase inhibitor, did not inhibit the effects of EGCG. EGCG-induced effects on pacemaker currents were not inhibited by glibenclamide, an ATP-sensitive K＋ channel blocker and TEA, a Ca2＋-activated K＋ channel blocker. Also, we found that EGCG inhibited the spontaneous [Ca2＋]i oscillations in cultured ICC. In conclusion, EGCG inhibited the pacemaker activity of ICC and reduced [Ca2＋]i oscillations by cAMP-, cGMP-, ATP-sensitive K+ channel-independent manner.
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KEYWORD
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(-)-epigallocatechin gallate (EGCG), Interstitial cells of Cajal (ICC), Pacemaker currents, Intestinal motility
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